Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology.
نویسندگان
چکیده
To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 48 2 شماره
صفحات -
تاریخ انتشار 2010